There isn't any backbone sampling so I typically uncover RMSD on the crystal framework to become < one.0. Unwind essentially will do explicit bb sampling So presents a decreased Electricity framework than my protocol but may also introduce the changes you observed. I'm pasting my standard possibilities file down below: https://finnjwjte.dm-blog.com/26855406/getting-my-พอตไฟฟ-า-to-work